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mouse myoblast cell line c2c12  (ATCC)


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    ATCC mouse myoblast cell line c2c12
    Mouse Myoblast Cell Line C2c12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+myoblast+c2c12+cells/C2C12/pm42841643-402-1-10
    Average 99 stars, based on 8700 article reviews
    mouse myoblast cell line c2c12 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Recombinant ADAMTS-1 promotes muscle regeneration accompanied by downregulation of Notch signaling
    Article Snippet: Primary skeletal muscle cells (cat. no. PCS-950-010; ATCC) were cultured in a complete expansion medium (cat. no. PCS-500-030; ATCC) using a primary skeletal muscle cell growth kit (cat. no. PCS-950-040; ATCC).

    Article Title: CpG island density predicts CBP/p300 dependency across 3D chromatin clusters
    Article Snippet: FP-RMS cell lines, RH4, RH41, RH30, and SCMC, were cultured in DMEM (Gibco, cat. #10313039) supplemented with 10% fetal bovine serum (FBS; Gibco, cat. #A3160402) and 1x penicillin-streptomycin-glutamine (Gibco, cat. #10378016).

    Article Title: Recombinant ADAMTS‑1 promotes muscle regeneration accompanied by downregulation of Notch signaling.
    Article Snippet: Primary skeletal muscle cells (cat. no. PCS‐950‐010; ATCC) were cultured in a complete expansion medium (cat. no. PCS‐500‐030; ATCC) using a primary skeletal muscle cell growth kit (cat. no. PCS‐950‐040; ATCC).

    Cell Culture:

    Article Title: ANXA11 suppression restores muscular function in the mdx mouse model of Duchenne muscular dystrophy (DMD).
    Article Snippet: 293T cells (ATCC Cat#CRL-3216) were cultured in DMEM medium (BasalMedia) supplemented with 10% fetal bovine serum (HAKATA) and 1% penicillin-streptomycin (HAKATA) at 5% CO2 and 37 °C. .. Mouse myoblast C2C12 cells (ATCC CatCRL-1772) were cultured in DMEM medium supplemented with 20% fetal bovine serum and 1% penicillin-streptomycin at 5% CO2 and 37 °C. ..

    Article Title: Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
    Article Snippet: .. Mouse myoblast C2C12 cells (American Type Culture Collection, VA, USA) and Human Skeletal Muscle Myoblasts (HSMM) (Zenbio, NC, USA) were cultured in high glucose Dulbecco’s modified Eagle growth medium (DMEM; Wisent, Canada) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. ..

    Modification:

    Article Title: Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
    Article Snippet: .. Mouse myoblast C2C12 cells (American Type Culture Collection, VA, USA) and Human Skeletal Muscle Myoblasts (HSMM) (Zenbio, NC, USA) were cultured in high glucose Dulbecco’s modified Eagle growth medium (DMEM; Wisent, Canada) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. ..



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    Cu-doped Prussian blue (CuPB) nanozymes protect <t>C2C12</t> myoblasts and H9c2 cardiomyocytes from H 2 O 2 -induced oxidative injury. (A and B) Representative fluorescence images and quantification of intracellular reactive oxygen species (ROS) in H 2 O 2 -injured C2C12 cells after Prussian blue (PB) or CuPB treatment, detected using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) probe. Scale bar: 50 μm. n = 5. (C and D) Representative terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining images and quantification of apoptotic C2C12 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (E) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of apoptosis-related genes ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in C2C12 cells after different treatments. n = 3. (F and G) Representative fluorescence images and quantification of intracellular ROS in H 2 O 2 -injured H9c2 cells after PB or CuPB treatment, detected using the DCFH-DA probe. Scale bar: 50 μm. n = 5. (H and I) Representative TUNEL staining images and quantification of apoptotic H9c2 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (J) qRT-PCR analysis of apoptosis-related gene expression ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in H9c2 cells after different treatments. n = 5.
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    Cu-doped Prussian blue (CuPB) nanozymes protect <t>C2C12</t> myoblasts and H9c2 cardiomyocytes from H 2 O 2 -induced oxidative injury. (A and B) Representative fluorescence images and quantification of intracellular reactive oxygen species (ROS) in H 2 O 2 -injured C2C12 cells after Prussian blue (PB) or CuPB treatment, detected using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) probe. Scale bar: 50 μm. n = 5. (C and D) Representative terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining images and quantification of apoptotic C2C12 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (E) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of apoptosis-related genes ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in C2C12 cells after different treatments. n = 3. (F and G) Representative fluorescence images and quantification of intracellular ROS in H 2 O 2 -injured H9c2 cells after PB or CuPB treatment, detected using the DCFH-DA probe. Scale bar: 50 μm. n = 5. (H and I) Representative TUNEL staining images and quantification of apoptotic H9c2 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (J) qRT-PCR analysis of apoptosis-related gene expression ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in H9c2 cells after different treatments. n = 5.
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    Image Search Results


    Cu-doped Prussian blue (CuPB) nanozymes protect C2C12 myoblasts and H9c2 cardiomyocytes from H 2 O 2 -induced oxidative injury. (A and B) Representative fluorescence images and quantification of intracellular reactive oxygen species (ROS) in H 2 O 2 -injured C2C12 cells after Prussian blue (PB) or CuPB treatment, detected using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) probe. Scale bar: 50 μm. n = 5. (C and D) Representative terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining images and quantification of apoptotic C2C12 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (E) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of apoptosis-related genes ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in C2C12 cells after different treatments. n = 3. (F and G) Representative fluorescence images and quantification of intracellular ROS in H 2 O 2 -injured H9c2 cells after PB or CuPB treatment, detected using the DCFH-DA probe. Scale bar: 50 μm. n = 5. (H and I) Representative TUNEL staining images and quantification of apoptotic H9c2 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (J) qRT-PCR analysis of apoptosis-related gene expression ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in H9c2 cells after different treatments. n = 5.

    Journal: Research

    Article Title: Doping-Engineered Proangiogenic Nanozymes Orchestrate Ischemic Tissue Regeneration via Cytoprotection and Revascularization

    doi: 10.34133/research.1260

    Figure Lengend Snippet: Cu-doped Prussian blue (CuPB) nanozymes protect C2C12 myoblasts and H9c2 cardiomyocytes from H 2 O 2 -induced oxidative injury. (A and B) Representative fluorescence images and quantification of intracellular reactive oxygen species (ROS) in H 2 O 2 -injured C2C12 cells after Prussian blue (PB) or CuPB treatment, detected using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) probe. Scale bar: 50 μm. n = 5. (C and D) Representative terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining images and quantification of apoptotic C2C12 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (E) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of apoptosis-related genes ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in C2C12 cells after different treatments. n = 3. (F and G) Representative fluorescence images and quantification of intracellular ROS in H 2 O 2 -injured H9c2 cells after PB or CuPB treatment, detected using the DCFH-DA probe. Scale bar: 50 μm. n = 5. (H and I) Representative TUNEL staining images and quantification of apoptotic H9c2 cells following H 2 O 2 injury with PB or CuPB treatment. Scale bar: 50 μm. n = 5. (J) qRT-PCR analysis of apoptosis-related gene expression ( Bcl2 , Caspase3 , Caspase9 , and Bax ) in H9c2 cells after different treatments. n = 5.

    Article Snippet: The rat cardiomyocyte cell line (H9c2) was obtained from Procell Life Science & Technology Co., Ltd. (China), and the mouse myoblast cell line (C2C12) was purchased from Beijing Zhongyuan Heju Biotechnology Co., Ltd., the authorized American Type Culture Collection distributor in China (CRL1772).

    Techniques: Fluorescence, End Labeling, TUNEL Assay, Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression